Anti-inflammatory Activity of Ethanol Extract of Sappan Wood (Caesalpinia sappan L.) on Mice Infected by Staphylococcus aureus and Escherichia coli

Rohmah, Martina Kurnia and Taniasari, Nungky and Olevianingrum, Melawati and Kinanti, Resti and Wulansari, Cintia Anti-inflammatory Activity of Ethanol Extract of Sappan Wood (Caesalpinia sappan L.) on Mice Infected by Staphylococcus aureus and Escherichia coli. In: International Conference on Health Innovation.

	Text Martina - Immunomodulatory.pdf Download (430kB)
	Text Similarity Check - Anti-inflammatory Activity of Ethanol Extract of Sappan Wood (Caesalpinia sappan L.) on (1).pdf Download (2MB)

Abstract

Background: Sappan wood (Caesalpinia sappan L.) is distributed in Southeast Asia and used as traditional medicine. Sappan wood contains phytochemical properties such as brazilin, brazilein, Sappanone B, protosappanin etc. This study aims to investigate about the anti- inflammatory activity of ethanol extract of Sappan wood in mice infected by Staphylococcus aureus and Escherichia coli. Methods: Seven group of mice were treated respectively such as normal control, negative control (placebo), positive control (immune booster), and 96 % ethanol extract of Sappan wood (SWEE) 25, 50, 100, and 200 mg/kg body weight that was given for 7 days after infection. Blood samples was taken to measure the C-reactive protein (CRP) level and phagocytic index. Molecular docking studies were carried out using a webserver. Results: The results showed that ethanol extract of Sappan wood decrease CRP level and increase phagocytic index until 48 mg/L and 1.54 folds (Staphylococcus aureus infection) and 12 mg/L and 4.62 folds (Escherichia coli infection). The molecular docking showed that brazilin has the potential to bind to TNFα, and IL-1β with total energy binding respectively - 5,05, and -3,14 kcal/mol. The estimation inhibition constant (Ki) of brazilin into TNFα, and IL-1β respectively 199,52 and 4,99 µM. Brazilin has less potential to bind to IL-6. Conclusion: The ethanol extract of Sappan wood has anti-inflammatory activity through decreasing CRP level and increasing phagocytic index. Brazilin as a major compound of Sappan wood can bind and inhibit pro-inflammatory cytokine including TNFα and IL-1β. Keywords: Anti-inflammation, CRP, phagocytic index, brazilin

Item Type:	Conference or Workshop Item (Paper)
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User:	Triani Febriana Triani
Date Deposited:	02 May 2024 00:36
Last Modified:	02 May 2024 00:36
URI:	http://repository.uam.ac.id/id/eprint/1076

Actions (login required)

View Item